Diagnostic value of circulating microRNAs for esophageal cancer: a meta-analysis based on Asian data

Background and objective: esophageal cancer (EC) is one of the most common gastrointestinal malignant diseases. We conducted a comprehensive meta-analysis to explore the clinical applicability of circulating microRNA for the diagnosis of EC. Methods: as of September 10, 2021, a comprehensive literature search was conducted on PubMed, Embase, Web of Science, Cochrane Library, Wanfang Database, and China National Knowledge Infrastructure (CNKI) to identify eligible studies. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were pooled to evaluate the test performance. The potential sources of heterogeneity were analyzed by subgroup analysis. Deeks' funnel plot was used to assess publication bias. Results: 85 studies from 50 articles were included in the current meta-analysis. The overall pooled sensitivity was 0.82 (95 % CI, 0.79-0.84), specificity was 0.84 (95 % CI, 0.81-0.86), PLR was 4.9 (95 % CI, 4.2-5.9), NLR was 0.22 (95 % CI, 0.19-0.25), DOR was 22 (95 % CI, 17-29) and AUC was 0.89 (95 % CI, 0.86-0.92), respectively. Subgroup analysis suggested that miRNA clusters with a large sample size showed better diagnostic accuracy. Publication bias was not found. Conclusions: circulating miRNAs can be used as a potential non-invasive biomarker for the diagnosis of EC in Asian populations. (AU)

Diagnostic value of circulating microRNAs for esophageal cancer: a meta-analysis based on Asian data.

BACKGROUND AND OBJECTIVE: esophageal cancer (EC) is one of the most common gastrointestinal malignant diseases. We conducted a comprehensive meta-analysis to explore the clinical applicability of circulating microRNA for the diagnosis of EC. METHODS: as of September 10, 2021, a comprehensive literature search was conducted on PubMed, Embase, Web of Science, Cochrane Library, Wanfang Database, and China National Knowledge Infrastructure (CNKI) to identify eligible studies. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were pooled to evaluate the test performance. The potential sources of heterogeneity were analyzed by subgroup analysis. Deeks' funnel plot was used to assess publication bias. RESULTS: 85 studies from 50 articles were included in the current meta-analysis. The overall pooled sensitivity was 0.82 (95 % CI, 0.79-0.84), specificity was 0.84 (95 % CI, 0.81-0.86), PLR was 4.9 (95 % CI, 4.2-5.9), NLR was 0.22 (95 % CI, 0.19-0.25), DOR was 22 (95 % CI, 17-29) and AUC was 0.89 (95 % CI, 0.86-0.92), respectively. Subgroup analysis suggested that miRNA clusters with a large sample size showed better diagnostic accuracy. Publication bias was not found. CONCLUSIONS: circulating miRNAs can be used as a potential non-invasive biomarker for the diagnosis of EC in Asian populations.

Circulating microRNAs as Potential Biomarkers for the Diagnosis of Endometrial Cancer: a Meta-Analysis.

Endometrial cancer (EC) is the most common female genital tract malignancy worldwide. Many investigators have confirmed the possibility of using circulating miRNAs to diagnose EC; however, the results were inconsistent. Therefore, we performed the current meta-analysis to systematically evaluate the diagnostic value of circulating miRNAs in EC. We carefully searched relevant articles published prior to February 15, 2022 in the databases of PubMed, Embase, Web of Science, Cochrane Library, Wanfang database, and China National Knowledge Infrastructure (CNKI) based on PRISMA statement. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were calculated to test the diagnostic accuracy. Furthermore, subgroup analyses were performed to identify the potential sources of heterogeneity, and the Deeks' funnel plot asymmetry test was used to evaluate the potential publication bias. Twenty-one studies from 12 articles including a total of 2305 participants (1341 EC patients and 964 controls) were included in the current diagnostic meta-analysis. The overall pooled results of miRNA for EC diagnosis were: sensitivity, 0.84 (95% CI: 0.79-0.88); specificity, 0.87 (95% CI: 0.79-0.91); PLR, 6.3 (95% CI: 3.9-10.0); NLR, 0.18 (95% CI: 0.13-0.25); DOR, 35 (95% CI: 17-71); and AUC was 0.91 (95% CI: 0.89-0.94). Subgroup analysis suggested that miRNA cluster, serum type, and large sample sizes showed a better diagnostic accuracy. Moreover, there was no significant publication bias. Circulating miRNAs have great potential as novel non-invasive biomarkers for EC diagnosis.

The epidemiological status of osteoporotic hip fractures: A bicentric comparative and retrospective study.

BACKGROUND: Osteoporotic hip fractures have posed a significant burden to society, and more epidemiological data is required. OBJECTIVE: To compare the epidemiological differences of hip fracture patients in Spain and China. METHOD: This was a retrospective comparative study. Comparisons were made in terms of morbidity, demographic and anthropometric characteristics, length of stay, cost of hospitalization, and mortality by consulting the medical histories of osteoporotic hip fractures in two hospitals. The t test was used for measurement data, and the X2 test was used for count data. The difference is statistically significant when p< 0.05. RESULTS: A total of 757 patients were enrolled in this study, with 426 from Virgen Macarena University Hospital (HUVM) and 331 from Xi'an Daxing Hospital (XDH). The average age was 81.4 ± 9.26 and 76.0 ± 8.08 years; the proportion of women was 74.9% and 68.0%, respectively. The incidence of osteoporotic hip fractures in Seville residents over 50 years old was approximately 239 per 100,000 residents, compared to 158 per 100,000 residents in Xi'an. The timing of surgery in Spanish patients was significantly longer than in Chinese patients, 78.7 ± 48.2 vs. 60.7 ± 43.1 hours, p= 0.000. There were 81 deaths in Spain and 43 deaths in China during the one-year follow-up period (p= 0.026). CONCLUSIONS: In terms of incidence, demographics, surgical methods, and mortality, there are significant differences between hip fracture patients in Seville, Spain and Xi'an, China.

Diagnostic accuracy of circulating microRNA in hepatitis B virus-related hepatocellular carcinoma: a meta-analysis based on Asian data

Background and aim: hepatitis B virus (HBV) is the main risk factor for hepatocellular carcinoma (HCC). We performed a meta-analysis based on Asian data to evaluate the diagnostic accuracy of circulating microRNA as a non-invasive biomarker in the diagnosis of HBV-related HCC.Methods: a comprehensive literature search (updated to May 12, 2021) in PubMed, Embase, Web of Science, Wanfang Database, and China National Knowledge Infrastructure (CNKI) was performed to identify eligible studies. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) for diagnosing HBV-related HCC were pooled in this meta-analysis. A subgroup analysis was performed to explore heterogeneity, and Deeks' funnel plot was used to assess publication bias.Results: a total of 19 articles including 32 studies were included in the current meta-analysis. The overall sensitivity, specificity, PLR, NLR, DOR and AUC were 0.83 (95 % CI: 0.79 to 0.87), 0.78 (95 % CI: 0.73 to 0.83), 3.9 (95 % CI: 3.0 to 4.9), 0.21 (95 % CI: 0.16 to 0.27), 18 (95 % CI: 12 to 27) and 0.88 (95 % CI: 0.85 to 0.91), respectively. Subgroup analysis shows that miRNA clusters with a large sample size showed better diagnostic accuracy. Although there is no publication bias, the research still has some limitations.Conclusions: circulating miRNAs could serve as a potential non-invasive biomarker in diagnosing HBV-related HCC in Asian populations. (AU)

Plicatura artroscópica del retináculo medial combinada con liberación del retináculo lateral en el tratamiento de la luxación rotuliana traumática / Arthroscopic medial retinaculum plication combined with lateral retinaculum release in the treatment of traumatic patellar dislocation

Objetivo: El objetivo fue analizar el efecto curativo de la plicatura artroscópica del retináculo medial (PARM) combinada con la liberación del retináculo lateral (LRL) para el tratamiento de la luxación rotuliana traumática. Métodos: Se recogieron retrospectivamente los datos de los pacientes con luxación rotuliana traumática desde enero de 2015 hasta diciembre de 2020. El grupo de control fueron los pacientes que recibieron reconstrucción del ligamento patelomeniscal medial (LPMM); el grupo de observación fueron los con PARM en forma de abanico combinada con LRL. La longitud de la incisión, la función de la rodilla, los parámetros del examen de TC y el costo hospitalario se compararon entre los dos grupos antes y después de la cirugía. Resultados: El grupo de control y el grupo de observación incluyeron 35 y 39 pacientes, con una edad pro-medio de 18,8±5,3 años y 18,1±5,9 años, respectivamente. Los parámetros de TC preoperatorios y la puntuación de Lysholm y la puntuación de Kujala de los dos grupos no fueron estadísticamente significativos. Ambos grupos lograron buenos resultados posoperatorios. No hubo diferencias significativas en los parámetros de TC posoperatorios y la puntuación de Kujala, pero el grupo de observación fue más dominante en la longitud de la incisión, la prueba de movilidad rotuliana, la puntuación de Lysholm y el costo hospitalario (p < 0,05). Conclusión: La PARM combinada con LRL en el trata-miento de la luxación rotuliana traumática tiene las ventajas de menos trauma, baja tasa de recurrencia, bajo costo y eficacia confiable a corto plazo. (AU)

Objetive: The aim of this study was to analyze the cu-rative effect of arthroscopic medial retinaculum plication (MRP) combined with lateral retinacu-lum release (LRR) for the treatment of traumatic patellar dislocation. Methods: The data of patients with traumatic patellar dislocation treated from January 2015 to December 2020 were retrospectively collected. The con-trol group was the patients who received medial patellomeniscal ligament (MPFL) reconstruction; the observation group was the patients with ar-throscopic fan-shaped MRP combined with LRR. The incision length, knee function, CT examina-tion parameters, and hospital cost were compared between the two groups before and after surgery. Results: The control group and the observation group included 35 and 39 patients, with an average age of 18.8±5.3 years and 18.1±5.9 years, respec-tively. The preoperative CT parameters, and Ly-sholm score and Kujala score of the two groups of patients were not statistically significant. Both groups of patients achieved good results after sur-gery. There was no significant difference in post-operative CT parameters and Kujala score, but the observation group was more dominant in inci-sion length, patellar mobility test, Lysholm score, and hospital cost (p < 0.05). Conclusion: Arthroscopic MRP combined with LRR in the treatment of traumatic patellar dislocation has the advantages of less trauma, low recurrence rate, low cost, and reliable short-term efficacy. (AU)

Análisis de los factores de riesgo de mortalidad al año en pacientes con fractura de cadera osteoporótica en España: un estudio observacional prospectivo / Analysis of risk factors for mortality at one year in patients with osteoporotic hip fracture in Spain: a prospective observational study

Objetivo: Analizar los factores de riesgo que inciden en la mortalidad al año de las fracturas de cadera osteoporóticas en España. Materiales y métodos: Fue un estudio observacional prospectivo. Los pacientes con fractura de cadera que fueron intervenidos quirúrgicamente en el Hospital Universitario Virgen Macarena de Sevilla, España del1 de enero de 2017 al 31 de diciembre de 2017. Se recopiló la información requerida a través de registros de historia clínica y seguimiento. Se realizó un análisis de factor único para descartar los factores con p<0,1, y luego estos factores se incluyeron en el análisis de regresión de Cox para averiguar los factores de riesgo independientes para la mortalidad posoperatoria al año. Resultados: Se incluyeron un total de 426 participantes, 107 hombres (25,1%) y 319 mujeres (74,9%). Durante el seguimiento de un año fallecieron 81 casos (19,0%). Hubo 7 factores de riesgo independientes relacionados con la tasa de mortalidad al año de las fracturas de cadera osteoporóticas en España: Los factores de riesgo inmodificables incluyeron los niveles de actividad previos a la lesión, ASA grado 4 y enfermedad pulmonar combina-da. Los factores de riesgo prevenibles incluyeron complicaciones respiratorias, complicaciones cardiovasculares, complicaciones cerebrovasculares, y trombosis venosa. Conclusión: La tasa de mortalidad de las fracturas de cadera osteoporóticas en España es relativamente alta, pero mediante la prevención activa de las complicaciones postoperatorias, la tasa de mortalidad puede disminuir. (AU)

Objetive: Aanalyze the risk factors that affect the one-year mortality of osteoporotic hip fractures in Spain. Materials and methods: This was a prospective observational study. Participants came from patients with hip fractures who underwent surgery at the Virgen Macarena University Hospital in Seville, Spain from January 1, 2017 to December 31, 2017. The required infor-mation was collected through medical history re-cords and follow-up. A single factor analysis was performed to screen out the factors with p<0.1, and then these factors were brought into the Cox regression analysis to find out the independent risk factors for the one-year postoperative mortality. Results: A total of 426 participants were included, 107 males (25.1%) and 319 females (74.9%). During the one-year follow-up, 81 cases (19.0%) died. There were 7 independent risk factors related to the one-year mortality rate of osteoporotic hip fractures in Spain: Unchangeable risk factors in-cluded pre-injury activity levels, ASA grade 4 , and combined lung disease. Preventable risk factors included respiratory complications, cardiovascu-lar complications cerebrovascular complications, and venous thrombosis. Conclusion: The mortality rate of osteoporotic hip frac-tures in Spain is relatively high, but through active prevention of postoperative complications, the mortality rate may decrease. (AU)

Diagnostic accuracy of circulating microRNA in hepatitis B virus-related hepatocellular carcinoma: a meta-analysis based on Asian data.

BACKGROUND AND AIM: hepatitis B virus (HBV) is the main risk factor for hepatocellular carcinoma (HCC). We performed a meta-analysis based on Asian data to evaluate the diagnostic accuracy of circulating microRNA as a non-invasive biomarker in the diagnosis of HBV-related HCC. METHODS: a comprehensive literature search (updated to May 12, 2021) in PubMed, Embase, Web of Science, Wanfang Database, and China National Knowledge Infrastructure (CNKI) was performed to identify eligible studies. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) for diagnosing HBV-related HCC were pooled in this meta-analysis. A subgroup analysis was performed to explore heterogeneity, and Deeks' funnel plot was used to assess publication bias. RESULTS: a total of 19 articles including 32 studies were included in the current meta-analysis. The overall sensitivity, specificity, PLR, NLR, DOR and AUC were 0.83 (95 % CI: 0.79 to 0.87), 0.78 (95 % CI: 0.73 to 0.83), 3.9 (95 % CI: 3.0 to 4.9), 0.21 (95 % CI: 0.16 to 0.27), 18 (95 % CI: 12 to 27) and 0.88 (95 % CI: 0.85 to 0.91), respectively. Subgroup analysis shows that miRNA clusters with a large sample size showed better diagnostic accuracy. Although there is no publication bias, the research still has some limitations. CONCLUSIONS: circulating miRNAs could serve as a potential non-invasive biomarker in diagnosing HBV-related HCC in Asian populations.

El impacto del transporte público en las fracturas de cadera: una comparación entre dos grandes ciudades de España y China / The impact of public transportation facilities on hip fractures: a comparison between two major cities in Spain and China

Objetivo: Explorar la influencia de los medios de transporte público en diferentes países en las fracturas de cadera. Materiales y métodos: Los sujetos del estudio fueron pacientes con fractura de cadera intervenidos en el Xi’an Daxing Hospital, China y en el Hospital Universitario Virgen Macarena de Sevilla, España del 1 de enero al 30 de diciembre de 2017. Sexo, edad, el lugar de la caída, el tiempo de la caída, la clasificación de ASA, el alcance principal de las actividades antes y después de la lesión, las razones para restringir las salidas y la satisfacción con las instalaciones de transporte público del paciente se recopilaron mediante la consulta de registros médicos y el seguimiento ambulatorio durante un año. Resultados: Se incluyeron 178 pacientes en Sevilla (España), con una edad media de 83,2 ± 6,9 años, 45 varones (25,3%) y 131 mujeres (74,7%); 139 pacientes en Xi’an (China), con una edad media de 77,8 ± 8,2 años, y 47 hombres (33,8%), 92 mujeres (66,2%). La proporción de pacientes españoles que se lesionó al aire libre y durante el día fue significativamente mayor que la de los pacientes chinos (p = 0,000 y p = 0,017, respectivamente). El alcance de las actividades de los pacientes españoles antes y después de la lesión fue significativamente mayor que el de los pacientes chinos (p = 0,000 y p = 0,000, respectivamente). Entre las razones que impiden que los pacientes salgan antes de la lesión, el factor más importante para los pacientes en Xi’an fueron las instalaciones de transporte público, mientras que en Sevilla fue su propia debilidad y movilidad inconveniente. En la encuesta de satisfacción con los medios de transporte público, la satisfacción de los pacientes españoles fue significativamente superior a la de los pacientes chinos (p = 0,000). Conclusión: El transporte público tiene un gran impacto en los viajes de las personas mayores...(AU)

Objetive: To explore the influence of public transportation facilities in different countries on hip fractures. Materials and methods: The subjects of the study were hip fracture patients who underwent operations at the Daxing Hospital in Xi’an, China and the Hospital Universitario Virgen Marcarena in Seville, Spain from January 1 to December 30, 2017. The patient’s gender, age, place of injury, time of injury, ASA classification, main scope of activities before and after injury, reasons for restricting going out, and satisfaction with public transportation facilities were collected by consulting medical records and outpatient follow-up for one year. Results: A total of 178 patients were enrolled in Seville (Spain), with an average age of 83.2±6.9 years, 45 males (25.3%), and 131 females (74.7%); a total of 139 patients in Xi’an (China), with an average age of 77.8±8.2 years old, and 47 males (33.8%), 92 women (66.2%). The proportion of Spanish patients who were injured outdoors and during the day was significantly greater than that of Chinese patients (p=0.000 and p=0.017, respectively). The scope of activities of Spanish patients before and after injury was significantly greater than that of Chinese patients (p=0.000 and p=0.000, respectively). Among the reasons that restrict patients from going out before injury, the biggest factor for patients in Xi’an was public transportation facilities, while in Seville was its own weakness and inconvenient mobility. In the survey of satisfaction with public transportation facilities, the satisfaction of Spanish patients was significantly higher than that of Chinese patients (p=0.000). Conclusion: Public transportation has a great impact on the travel of the elderly. Municipal governments of various countries should pay attention to and...(AU)

The Potential Diagnostic Accuracy of Let-7 Family for Cancer: A Meta-Analysis.

BACKGROUND: Cancer is a global public health problem affecting human health. Early stage of cancer diagnosis, when it is not too large and has not spread is important for successful treatment. Many researchers have proposed that the let-7 microRNA family can be used as a biomarker for cancer diagnosis. The aim of this meta-analysis is to evaluate whether let-7 family can be used as a diagnostic tool for cancer patients. METHODS: We conducted a comprehensive literature search on PubMed, EMBASE, Web of Science, Cochrane Library, Google Scholar, China National Knowledge Infrastructure (CNKI) and Wanfang database, updated to October 23, 2020. A random effects model was used to pool the sensitivity and specificity. Besides, we measured the diagnostic value using positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve (AUC) were pooled. In addition, meta-regression and subgroup analysis were performed to explore the possible sources of heterogeneity, and Deeks' funnel chart was used to assess whether there was publication bias. RESULTS: 31 studies from 15 articles were included in the current meta-analysis. The overall sensitivity, specificity, PLR, NLR, DOR and AUC were 0.80 (95% CI: 0.75-0.85), 0.81 (95% CI: 0.74-0.86), 4.2 (95% CI: 2.9-5.9), 0.24 (95% CI: 0.19-0.32), 17 (95% CI: 10-29) and 0.87 (95% CI: 0.84-0.90), respectively. Subgroup analysis shows that the let-7 family cluster of serum type showed a better diagnostic accuracy of cancer, especially the breast cancer. Although there is no publication bias, it still has some limitations. CONCLUSIONS: let-7 family can be considered as a promising non-invasive diagnostic biomarker for cancer.

Rehabilitation guidance for hip fracture patients during the COVID-19 pandemic using chat software: A new model.

BACKGROUND: Hip fractures are serious fractures for the elderly. The rehabilitation of patients with hip fractures has been greatly affected by the coronavirus disease 2019 (COVID-19) pandemic. OBJECTIVE: We have piloted a new model for tracking patients and providing rehabilitation guidance that uses WeChat. The purpose of this study is to explore the role of chat software in rehabilitation guidance for hip fracture patients during COVID-19. METHODS: Patients treated for hip fractures from February 1 to April 30, 2020 were randomly divided into a control group and an observation group. The control group was given conventional discharge guidance, while the observation group also followed up the patients using WeChat to guide the exercise. Satisfaction, the Harris Hip Score, complications and the mortality of the two groups after discharge were compared. RESULTS: The incidence of complications and mortality in the observation group were significantly lower than in the control group: p= 0.022 and p= 0.048, respectively. The Harris Hip Score and satisfaction were significantly better than the control group's: p= 0.000 and p= 0.007, respectively. CONCLUSION: During the COVID-19 pandemic, it is very helpful to use WeChat software or other social software with similar functions (such as WhatsApp and Facebook) to guide the rehabilitation of hip fractures.

The Potential Diagnostic Accuracy of Circulating MicroRNAs for Leukemia: A Meta-Analysis.

BACKGROUND: Leukemia is a common malignant disease in the human blood system. Many researchers have proposed circulating microRNAs as biomarkers for the diagnosis of leukemia. We conducted a meta-analysis to evaluate the diagnostic accuracy of circulating miRNAs in the diagnosis of leukemia. METHODS: A comprehensive literature search (updated to October 13, 2020) in PubMed, EMBASE, Web of Science, Cochrane Library, Wanfang database and China National Knowledge Infrastructure (CNKI) was performed to identify eligible studies. The sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) for diagnosing leukemia were pooled for both overall and subgroup analysis. The meta-regression and subgroup analysis were performed to explore heterogeneity and Deeks' funnel plot was used to assess publication bias. RESULTS: 49 studies from 22 publications with a total of 3,489 leukemia patients and 2,756 healthy controls were included in this meta-analysis. The overall sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio and area under the curve were 0.83, 0.92, 10.8, 0.18, 59 and 0.94, respectively. Subgroup analysis shows that the microRNA clusters of plasma type could carry out a better diagnostic accuracy of leukemia patients. In addition, publication bias was not found. CONCLUSIONS: Circulating microRNAs can be used as a promising noninvasive biomarker in the early diagnosis of leukemia.

Potential diagnostic value of circulating miRNA for multiple myeloma: A meta-analysis.

BACKGROUND: Multiple myeloma (MM) is the second incurable hematological malignancy. In recent years, due to the rise of microRNA (miRNA), many scholars have participated in the study of its value in the diagnosis of MM, and have obtained good but inconsistent results. Therefore, in order to determine the role of miRNA in the early diagnosis of MM, we performed this meta-analysis. METHODS: We searched for related studies including PubMed, Web of Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang Database as of July 20, 2020 to conduct this meta-analysis. To improve the accuracy, the quality assessment of Diagnostic Accuracy Study 2 (QUADAS-2) was used. We also applied random effects models to summarize sensitivity and specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve (AUC) to measure diagnostic values, and subgroup analysis used to discover potential sources of heterogeneity. RESULTS: We finally collected 32 studies from 15 articles that included a total of 2053 MM patients and 1118 healthy controls in this meta-analysis. The overall sensitivity, specificity, PLR, NLR, DOR and AUC were 0.81, 0.85, 5.5, 0.22, 25 and 0.90, respectively. Subgroup analysis shows that the down-regulation of microRNA clusters with larger samples size of plasma type could carry out a better diagnostic accuracy of MM patients. In addition, publication bias was not found. CONCLUSIONS: Circulating miRNA could be a potential non-invasive biomarker for early diagnosis of MM. However, multi-center, more rigorous, and larger-scale studies are needed to verify our conclusions.

Potential diagnostic value of miRNAs in peripheral blood for osteosarcoma: A meta-analysis.

Osteosarcoma (OS) is one of the most common primary malignant tumors in adolescents. In recent years, multiple studies have reported the value of miRNAs in the diagnosis of OS, but the results were very different from each other. Therefore, we conducted this meta-analysis to determine the accuracy of miRNAs in the diagnosis of OS. The meta-analysis searched for relevant researches including PubMed, EMBASE, Web of Science, Wanfang database and China National Knowledge Infrastructure (CNKI) as of June 1, 2020. We used the quality assessment of Diagnostic Accuracy Study 2 (QUADAS-2) to score the quality of each study. A random effects model was used to pool the sensitivity and specificity. We measured the diagnostic value using positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the curve (AUC). Subgroup and meta-regression analysis were used to find potential sources of heterogeneity. The meta-analysis finally included 31 articles about 2634 OS patients and 1715 healthy controls. The pooled estimations showed that the circulating miRNAs has a high accuracy in diagnosing OS, with a sensitivity of 0.79, specificity of 0.89, PLR of 7.3, NLR of 0.23, DOR of 31, and AUC of 0.90. In addition, subgroup and meta-regression analysis showed that miRNA clusters have higher diagnostic accuracy than single miRNA, and miRNAs in plasma were more reliable than those in serum. In conclusion, peripheral blood miRNA is a potential noninvasive biomarker to assist in the early diagnosis of OS, especially young patients with bone pain and/or indeterminate radiology findings.